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Writer's pictureZoe Terzopoulou

New publication on 3D-printed scaffolds

We are happy to announce that our work with the title "Optimization of chitosan-gelatin-based 3D-printed scaffolds for tissue engineering and drug delivery applications" has been published in International Journal of Pharmateutics!



In this paper, we present an innovative approach to the development of 3D-printed scaffolds utilizing chitosan-gelatin (CS-Gel) based hydrogel inks, optimized for both tissue engineering and drug delivery applications. Our research focuses on enhancing the printability and structural stability of these scaffolds using pneumatic-based extrusion printing technology.


The study explored various chitosan-gelatin compositions, ultimately identifying an optimal formulation of 4% w/v chitosan and 1% w/v gelatin, which demonstrated superior print quality and extrusion performance. Post-printing stabilization using potassium hydroxide (KOH) resulted in self-supporting scaffolds with excellent structural integrity. Further testing confirmed the formation of stable polyelectrolyte complexes, as verified by FTIR, and demonstrated favorable swelling properties and stability across different pH environments.


Additionally, we incorporated Levofloxacin as a model antibiotic into the scaffolds and examined its release profile. While non-treated scaffolds exhibited rapid drug release, KOH-treated constructs provided a more controlled release, enhancing their potential for drug delivery. Antimicrobial tests against *E. coli* and *S. aureus* revealed significant bactericidal activity, making these scaffolds highly effective for biomedical applications.


Overall, our findings highlight the potential of these optimized 3D-printed scaffolds for tissue engineering and controlled drug release, paving the way for further development in regenerative medicine.


To read the full article, click here.

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